| Japanese |
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| Title | Ischemic preconditioning時の心筋間質内noradrenaline濃度上昇 -adenosine A1 receptor, protein kinase Cの関与- |
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| Subtitle | 原著 |
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| Authors | 湊口信也, 苅谷達也, 宇野嘉弘, 荒井正純, 大野道也, 大野嘉嗣, 橋本和明, 西田佳雄, 藤原久義 |
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| Organization | 岐阜大学医学部第二内科 |
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| Journal | 循環制御 |
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| Volume | 19 |
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| Number | 2 |
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| Page | 225-229 |
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| Year/Month | 1998/ |
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| Article | 原著 |
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| Publisher | 日本循環制御医学会 |
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| Abstract | 「要旨」ウサギにおいてischemic preconditioning(PC)のメカニズムとして, adenosine A1 receptor, protein kinase Cの他に, PC中に交感神経末端より放出されるnoradrenaline(NA)によるα1B receptor刺激が重要である. これらのブロッカーは単独で, PC効果をブロックできることから, 情報伝達系は, 心筋細胞レベルで相互に関連しているとされる. 我々は, 交感神経レベルでもこれらのメカニズムが起こっているという仮説を考えた. 日本白色種ウサギ(オス, 約2kg)の心臓前側壁枝の5分虚血・5分再灌流のPC前後に, microdialysis法を用い, 心筋間質内NA濃度をHPLCと電気化学法にて測定した. PC中に生ずる心筋間質内NA濃度の上昇が, adenosine A1 receptorおよびprotein kinase Cのブロッカー(8-p-sulfophenyl theophylline : 8-SPT, 7.5mg/kg, i.v., polymyxin B : PB, 24mg/kg, i.v.)でブロックされるか否かを検討した. |
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| Practice | 基礎医学・関連科学 |
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| Keywords | Ischemic preconditiony, 心筋間質内ノルアドレナリン, プロテインキナーゼC, Ischemic preconditioning, Cardiac interstitial noradrenaline, Adenosine, Protein kinase C, Rabbit |
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| English |
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| Title | Cardiac Interstitial Noradrenaline Level during Ischemic Preconditioning in Rabbits -Role of Adenosine Receptor and Protein Kinase C- |
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| Authors | Shinya Minatoguchi, Tatsuya Kariya, Yoshihiro Uno, Masazumi Arai, Michiya Ohno, Yoshitsugu Ohno, Kazuaki Hashimoto, Yoshio Nishida, Hisayoshi Fujiwara |
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| Organization | Second Department of Internal medicine, Gifu University School of Medicine |
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| Journal | Circulation Control |
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| Volume | 19 |
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| Number | 2 |
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| Page | 225-229 |
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| Year/Month | 1998/ |
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| Article | Original article |
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| Publisher | Japan Society of Circulation Control |
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| Abstract | Many mechanisms such as α1B-adrenoceptor, adenosine A1 receptor and protein kinase C involve in the ischemic preconditioning (PC) effect. It is considered they are cross-talking each other at the level of myocytes. However, the cross-talk may occur at the level of sympathetic nerve endings. We hypothesized that the increase in cardiac interstitial noradrenaline (NA) by PC is modified by the activation of adenosin receptors and protein kinase C. Methods : In 19 open chest Japanese white male rabbits anesthetized with pentobarbital, cardiac interstitial NA levels in the area at risk were measured before and during PC of 5 min ischemia and 5 min reperfusion in the presence or absence of adenosine blocker (8-p-sulphophenyl theophylline, 7.5mg/kg), protein kinase C inhibitor (polymyxin B, 24mg/kg) using microdialysis technique and HPLC. Results : PC increased cardiac interstitial NA levels and this increase was inhibited by adenosin blocker or protein kinase C inhibitor. Conclusions : PC increases cardiac interstitial NA through activation of adenosine receptor and protein kinase C. |
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| Practice | Basic medicine |
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| Keywords | Ischemic preconditioning, Cardiac interstitial noradrenaline, Adenosine, Protein kinase C, Rabbit |
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