| English |
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| Title | Molecular Genetic Technology Defines the Receptor Subtypes Responsible for Anesthetic and Cardiovascular Responses to alpha-2 Adrenergic Agonists |
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| Subtitle | |
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| Authors | Toshiki Mizobe*1, Takahiko Kamibayashi*2, Guo Tianzhi*2, Katsumi Harasawa*2, Mervyn Maze*2 |
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| Authors(kana) | |
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| Organization | *1Department of Anesthesiology, Kyoto Prefectural University of Medicine, *2Departments of Anesthesia, VAPAHCS and Stanford University School of Medicine |
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| Journal | Circulation Control |
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| Volume | 19 |
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| Number | 1 |
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| Page | 89-95 |
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| Year/Month | 1998/ |
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| Article | Original article |
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| Publisher | Japan Society of Circulation Control |
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| Abstract | [Abstract] The analgesic effects of α2 adrenergic agonists are mediated by activation of α2 adrenoceptor in the locus coeruleus (LC) and spinal cord (SC). It is not known which of the three α2 adrenoceptor subtypes is responsible for the analgesic effects. Using a genetargeting strategy, it is possible to determine their role in the analgesic action of dexmedetomidine, an α2 agonist. The guide cannulae were sited stereotactically in the LC or intrathecally in male Sprague-Dawley rats. The antinociceptive effect of dexmedetomidine was measured using the tail flick latency (TFL) response. Separate cohorts of rats were administered the ODNs directed against either the α2A or α2c adrenoceptor subtypes which are present in the CNS. Control rats were treated with scrambled ODNs or saline. The TFL response to dexmedetomidine was measured, before, immediately after, and 8 days after the ODN administration. Immediately following α2A ODN treatment, the TFL response to dexmedetomidine, either LC or SC, was significantly attenuated ; this recovered to the pretreatment value after 8 days. α2c ODN treatment did not change the analgesic response. The analgesic response to morphine was unaffected by α2A ODN treatment. These data strongly suggest that the α2A adrenoceptor subtype mediates the analgesic responses to dexmedetomidine both in the LC and in the SC. |
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| Practice | Basic medicine |
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| Keywords | Adrenoceptor, α2 adrenergic agonists, Analgesia, Dexmedetomidine |
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