| Japanese |
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| Title | イヌ敗血症性ショックモデルにおけるアドレノメデュリン遺伝子の発現分布 |
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| Subtitle | 原著 |
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| Authors | 小野紫 |
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| Authors(kana) | |
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| Organization | 帝京大学医学部麻酔科学講座 |
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| Journal | 循環制御 |
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| Volume | 19 |
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| Number | 1 |
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| Page | 99-105 |
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| Year/Month | 1998/ |
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| Article | 原著 |
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| Publisher | 日本循環制御医学会 |
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| Abstract | 「要旨」血管拡張性の強い降圧性ペプチドであるアドレノメデュリン(AM)の敗血症性ショックへの関与を明らかにするため, イヌを用いたショックモデルを作製し, AM遺伝子発現の組織分布およびその変化を, 臓器, 血管で詳細に検討した. 全身の主要動静脈において, AMのmRNAの発現は増加しており, AMがショック時の重篤な低血圧に関与すると考えられた. また循環器系以外の臓器でもAM遺伝子の変化が認められ, 特に視床下部, 十二指腸, 回腸, 腎臓皮質などで増加した. 以上の結果から, AMが複雑な敗血症性ショックの病態において, 循環動態以外の面でも関与する可能性が示唆された. |
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| Practice | 基礎医学・関連科学 |
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| Keywords | Adrenomedullin, Northern blot analysis, Endotoxin shock, Dog |
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| English |
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| Title | Gene Expression of Adrenomedullin in Canine Endotoxin Shock Model |
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| Subtitle | |
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| Authors | Yukari Ono |
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| Authors(kana) | |
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| Organization | Department of Anesthesiology, Teikyo University School of Medicine |
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| Journal | Circulation Control |
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| Volume | 19 |
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| Number | 1 |
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| Page | 99-105 |
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| Year/Month | 1998/ |
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| Article | Original article |
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| Publisher | Japan Society of Circulation Control |
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| Abstract | Gene expression of adrenomedullin (AM), a novel hypotensive peptide originally isolated from human pheochromocytoma, was investigated in the canine endotoxin shock model. Two mg/kg of lipopolysaccharide (LPS) or saline was administrated into the canine femoral vein, and the whole blood was collected every 1 hr to measure the plasma concentration of immunoreactive (ir) AM. Four hrs after LPS administration, the dogs were sacrificed by removing the blood, and tissues were collected immediately to extract RNA. Northern blot analysis showed detectable transcripts of AM in all of the organs and blood vessels. By LPS treatment, gene expression of AM increased in almost all of the blood vessels and another many tissues, e. g. the hypothalamus, the spinal cord, ileum, renal cortex, etc. In this study, endotoxin shock caused up to a 15-fold augmentation of plasma concentrations of ir-AM. The plasma concentrations of AM also increased in patients of sepsis. Though the origin of this increased AM has not been revealed, it is prospected that AM is secreted from the various tissues and blood vessels. And the augmentation of AM transcripts in all of the arteries and the veins is one of the factors for the hypotension with the septic shock. In addition, AM may play important roles in the extra-cardiovascular tissues in this syndrome. |
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| Practice | Basic medicine |
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| Keywords | Adrenomedullin, Northern blot analysis, Endotoxin shock, Dog |
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