| Japanese |
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| Title | 心筋梗塞によるリモデリング心のNCX発現量増加と二次的虚血によるアポトーシスとの関係 |
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| Authors | 齊藤直, 河原剛一 |
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| Organization | 北海道大学大学院情報科学研究科細胞情報工学研究室
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| Journal | 循環制御 |
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| Volume | 27 |
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| Number | 4 |
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| Page | 352-357 |
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| Year/Month | 2006/12 |
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| Article | 原著 |
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| Publisher | 日本循環制御医学会 |
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| Abstract | 「要旨」本研究では, 心筋梗塞によるリモデリング心が虚血, 再灌流傷害を受けた際の脆弱性とNCX発現動態との連関の解明を目的とした. 雄の成体ラットにおける心筋梗塞由来リモデリング心を摘出して灌流心を作製し, 虚血, 再灌流傷害負荷を与えた結果, 対照心と比較して有意なNCX1発現量の増加およびCaspase-3活性とCalpain活性の増加が確認された. このことは, 心筋梗塞による心室リモデリング時にNCX発現量が増加したため, その後の虚血, 再灌流傷害時にNCX機能逆転による細胞内Ca2+濃度上昇が促進され, その結果アポトーシスの誘導が促進された可能性を示唆している. 「緒言」心肥大, 心不全の心筋細胞において, 細胞内Ca2+濃度上昇がその病態に重要な役割を果たしていることが知られており1), 心肥大, 心不全心筋細胞における細胞内Ca2+濃度上昇の分子メカニズムを解明することは大変有意義なことである. 虚血傷害時にはNa+/Ca2+交換体(NCX)機能の逆転により細胞内Ca2+濃度が上昇することが示唆されている2). |
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| Practice | 基礎医学・関連科学 |
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| English |
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| Title | Pathophysiological Role of the Increase in the Expression of NCX Responsible for the Increased Vulnerability to Ischemia/Reperfusion in the Remodeled Hearts |
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| Authors | Tadashi Saitoh, Koichi Kawahara |
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| Organization | Laboratory of Cellular Cybernetics, Graduate School of Information Science and Technology, Hokkaido University
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| Journal | Circulation Control |
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| Volume | 27 |
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| Number | 4 |
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| Page | 352-357 |
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| Year/Month | 2006/12 |
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| Article | Original article |
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| Publisher | Japan Society of Circulation Control |
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| Abstract | The aim of this study is to evaluate the relationship between the increase in the expression of Na+/Ca2+ exchanger (NCX) and the increased vulnerability to ischemia/reperfusion in the remodeled hearts. The coronary artery of male adult rats was ligated. One week later, the hearts were removed and perfused with Krebs-Henseleit buffer (KHB) solution. Control hearts were perfused with KHB solution for 2 hours. Ischemic hearts were first perfused with KHB solution for 30 minutes, and then the perfusion was perfectly terminated and the hearts were immersed in saline for 30 min. The hearts were perfused again with KHB solution for 1 hour. Western blotting revealed that the expression of cardiac NCX in the remodeled hearts was significantly increased as compared with that in the non-remodeled hearts. Both the secondary ischemia-induced increases in the caspase-3 cleavage and the calpain activity were more intensively detected in the remodeled hearts than those in the non-remodeled hearts. These results suggest that the expression of cardiac NCX is increased in association with cardiac remodeling, and that the secondary ischemia might result in the marked increase in the concentration of intracellular Ca2+ via reversed NCX, leading to the increase in apoptotic cardiomyocytes in the remodeled hearts. |
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| Practice | Basic medicine |
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| Keywords | Na+/Ca2+ exchanger, remodeled heart, ischemia/reperfusion, apoptosis |
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